2X4F
The Crystal Structure of the human myosin light chain kinase LOC340156.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.880, 70.650, 96.570 |
| Unit cell angles | 90.00, 118.96, 90.00 |
Refinement procedure
| Resolution | 84.490 - 2.670 |
| R-factor | 0.19267 |
| Rwork | 0.190 |
| R-free | 0.24105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.526 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.130 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.040 | 0.650 |
| Number of reflections | 25080 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2M AMMONIUM_SULFATE, 2.5% W/V PEG 400, PH 8.0 HEPES |
