2X4D
Crystal structure of human phospholysine phosphohistidine inorganic pyrophosphate phosphatase LHPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.210, 39.410, 112.920 |
| Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.920 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hlt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.558 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.620 | 2.030 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 37847 | |
| <I/σ(I)> | 9.3 | 1.7 |
| Completeness [%] | 94.4 | 68.1 |
| Redundancy | 4.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.3M NA FORMATE, 25W/V PEG 3350 |
