2X10
Crystal structure of the complete EphA2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 41.920, 44.650, 93.420 |
Unit cell angles | 93.02, 98.22, 112.12 |
Refinement procedure
Resolution | 45.917 - 3.000 |
R-factor | 0.2388 |
Rwork | 0.236 |
R-free | 0.29860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3C8X 2e7h 1x5l |
RMSD bond length | 0.004 |
RMSD bond angle | 0.697 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.150 | 0.430 |
Number of reflections | 23406 | |
<I/σ(I)> | 6.5 | 1.6 |
Completeness [%] | 94.9 | 82.1 |
Redundancy | 1.71 | 1.22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1/3 V/V: PROTEIN, 1/3 V/V: 20 % POLYETHYLENE GLYCOL 6000, 1 M LICL, 0.1 M TRIS PH 8 1/3 V/V: 0.4 M NON-DETERGENT SULFOBETAINE 256 |