2X10

Crystal structure of the complete EphA2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-07
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	41.920, 44.650, 93.420
Unit cell angles	93.02, 98.22, 112.12

Refinement procedure

Resolution	45.917 - 3.000
R-factor	0.2388
Rwork	0.236
R-free	0.29860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3C8X 2e7h 1x5l
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.150	0.430
Number of reflections	23406
<I/σ(I)>	6.5	1.6
Completeness [%]	94.9	82.1
Redundancy	1.71	1.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1/3 V/V: PROTEIN, 1/3 V/V: 20 % POLYETHYLENE GLYCOL 6000, 1 M LICL, 0.1 M TRIS PH 8 1/3 V/V: 0.4 M NON-DETERGENT SULFOBETAINE 256