2X0B
Crystal structure of human angiotensinogen complexed with renin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 212.475, 212.475, 474.061 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.520 - 4.330 |
| R-factor | 0.31 |
| Rwork | 0.309 |
| R-free | 0.33400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wxw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_249)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.500 | 4.590 |
| High resolution limit [Å] | 4.350 | 4.350 |
| Rmerge | 0.130 | 0.860 |
| Number of reflections | 6793 | |
| <I/σ(I)> | 3.6 | 1 |
| Completeness [%] | 97.2 | 89.2 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.6-2.2M AS, 0.1M MES, PH6 |
