2X04
Crystal structure of the PABC-TNRC6C complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.110, 72.230, 37.040 |
| Unit cell angles | 90.00, 119.76, 90.00 |
Refinement procedure
| Resolution | 29.380 - 1.490 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18600 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 27112 | |
| <I/σ(I)> | 17 | 2.87 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 3.66 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M NA-ACETATE PH 4.6, 200 MM AMMONIUM SULFATE, 30% (W/V) PEG 4000 |
