2WYX
Neutron structure of a class A Beta-lactamase Toho-1 E166A R274N R276N triple mutant
Experimental procedure
Source type | NUCLEAR REACTOR |
Source details | ILL BEAMLINE LADI III |
Synchrotron site | ILL |
Beamline | LADI III |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-03 |
Wavelength(s) | 3.7 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 72.993, 72.993, 98.365 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.409 - 2.100 |
R-factor | 0.226 |
Rwork | 0.223 |
R-free | 0.25790 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.100 |
Data reduction software | LAUEGEN |
Data scaling software | LAUEGEN |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.177 | 0.241 |
Number of reflections | 15814 | |
<I/σ(I)> | 10.4 | 7 |
Completeness [%] | 88.6 | 78 |
Redundancy | 8.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |