2WYO

Trypanosoma brucei glutathione synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	92.590, 125.150, 243.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.460 - 3.150
R-factor	0.2063
Rwork	0.203
R-free	0.27671
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m0w
RMSD bond length	0.012
RMSD bond angle	1.426
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0097)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	3.320
High resolution limit [Å]	3.150	3.150
Rmerge	0.120	0.510
Number of reflections	49689
<I/σ(I)>	9	2.7
Completeness [%]	99.9	100
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1