2WYH
Structure of the Streptococcus pyogenes family GH38 alpha-mannosidase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.573, 88.502, 134.688 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 127.000 - 1.900 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 152396 | |
| <I/σ(I)> | 14.4 | 1.95 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 12 MG/ML PROTEIN WITH 100 MM TRIS, PH 8.5, 1.5 M (NH4)2SO4, 12% V/V GLYCEROL. |
