2WYH
Structure of the Streptococcus pyogenes family GH38 alpha-mannosidase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-02 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.573, 88.502, 134.688 |
Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
Resolution | 127.000 - 1.900 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.20500 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.134 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.460 |
Number of reflections | 152396 | |
<I/σ(I)> | 14.4 | 1.95 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 12 MG/ML PROTEIN WITH 100 MM TRIS, PH 8.5, 1.5 M (NH4)2SO4, 12% V/V GLYCEROL. |