2WXF
The crystal structure of the murine class IA PI 3-kinase p110delta in complex with PIK-39.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.420, 65.010, 116.690 |
| Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
| Resolution | 38.950 - 1.900 |
| R-factor | 0.21052 |
| Rwork | 0.210 |
| R-free | 0.23986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rd0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.253 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.950 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.840 |
| Number of reflections | 80511 | |
| <I/σ(I)> | 16.31 | 1.78 |
| Completeness [%] | 98.5 | 96.3 |
| Redundancy | 6.79 | 6.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 20% (V/V) GLYCEROL, 10% (W/V) PEG 4K, 30 MM NANO3, 30 MM NA2HPO4, 30 MM (NH4)2SO4, 100 MM IMIDAZOLE PH 6.8 |
