2WWR
Crystal Structure of Human Glyoxylate Reductase Hydroxypyruvate Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.356, 116.080, 198.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.820 |
| R-factor | 0.22036 |
| Rwork | 0.217 |
| R-free | 0.27857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gcg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.180 | 0.790 |
| Number of reflections | 34609 | |
| <I/σ(I)> | 10.5 | 2 |
| Completeness [%] | 91.0 | 87.6 |
| Redundancy | 11.1 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M SODIUM CACODYLATE PH 6.75, 10% PEG 8K, 0.25M MAGNESIUM ACETATE TETRAHYDRATE |
