2WWK
Crystal structure of the Titin M10-Obscurin like 1 Ig F17R mutant complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.010, 37.800, 68.490 |
| Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
| Resolution | 31.270 - 1.700 |
| R-factor | 0.22073 |
| Rwork | 0.219 |
| R-free | 0.25881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wp3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.557 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.790 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 24975 | |
| <I/σ(I)> | 20.6 | 6 |
| Completeness [%] | 98.7 | 99.7 |
| Redundancy | 7.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M BIS-TRIS PH 5.5, 0.2 M AMMONIUM SULPHATE, 25% PEG 3350 |
