2WWE
Crystal structure of the phox homology domain of human phosphoinositide-3-kinase-C2-gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.420, 53.420, 75.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.710 - 1.250 |
| R-factor | 0.16596 |
| Rwork | 0.165 |
| R-free | 0.18398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2AR5 2iwl 1o7k 2v6v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.160 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 34900 | |
| <I/σ(I)> | 16.8 | 4.5 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M TRI-SODIUM CITRATE DIHYDRATE PH 5.5, 20% PEG3000 |
