2WVB
Structural and mechanistic insights into Helicobacter pylori NikR function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.010, 71.010, 227.795 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.789 - 1.900 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ca9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.550 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 27950 | |
| <I/σ(I)> | 6.4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.6 M NA FORMATE, 100 MM CITRATE PH 4.0 |
