2WV0
Crystal structure of the GntR-HutC family member YvoA from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-28 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.410, 137.080, 120.190 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 114.000 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.199 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.251 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.610 |
| Number of reflections | 249821 | |
| <I/σ(I)> | 9.2 | 1.9 |
| Completeness [%] | 96.4 | 96.6 |
| Redundancy | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M HEPES, PH 6.5, 1.26 M AMMONIUM SULPHATE, 0.111 M NDSB 256, 0.010 M NA-EDTA AND 1%(W/V) PEG 1000 |
