2WUT
Crystal structure of human myelin protein P2 in complex with palmitate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-22 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.330, 66.330, 101.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.377 - 1.850 |
R-factor | 0.1685 |
Rwork | 0.166 |
R-free | 0.20920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yiv |
RMSD bond length | 0.007 |
RMSD bond angle | 1.001 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.640 |
Number of reflections | 19658 | |
<I/σ(I)> | 24.2 | 2.3 |
Completeness [%] | 98.5 | 87.8 |
Redundancy | 6.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |