2WUS
Bacterial actin MreB assembles in complex with cell shape protein RodZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 81.813, 109.836, 112.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.256 - 2.900 |
| R-factor | 0.2017 |
| Rwork | 0.197 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jce |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.233 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 19032 | |
| <I/σ(I)> | 8.5 | 2.7 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 1000 MME, 478 MM SODIUM THIOCYANATE, 147 MM CACL2, 100 MM TRIS-HCL, PH 7.7, 6.5% ETOH |
