2WU7
Crystal Structure of the Human CLK3 in complex with V25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-16 |
| Detector | BRUKER |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.870, 62.200, 75.370 |
| Unit cell angles | 90.00, 97.97, 90.00 |
Refinement procedure
| Resolution | 28.940 - 2.250 |
| R-factor | 0.1934 |
| Rwork | 0.190 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | SADABS |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.320 | 2.290 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 19231 | |
| <I/σ(I)> | 7.26 | 2.12 |
| Completeness [%] | 99.8 | 96.2 |
| Redundancy | 6.59 | 4.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M (NH4)2SO4, 0.1M BIS-TRIS PH 5.5, 25% PEG 3350 |
