2WTT
Structure of the human p73 tetramerization domain (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.120, 84.000, 169.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.786 - 2.300 |
R-factor | 0.2333 |
Rwork | 0.230 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wqi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.176 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.700 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.090 | 0.354 |
Number of reflections | 36567 | |
<I/σ(I)> | 15.7 | 5.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.6 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 290 | SITTING DROP VAPOR DIFFUSION AT 17 DEGREE C. PROTEIN SOLUTION: 15 MG/ML IN 20 MM TRIS (PH 8.5), 50 MM NACL. CRYSTALLIZATION BUFFER: 0.1 M SODIUM CITRATE (PH 6.2), 40% PEG 600. |