2WSX
Crystal Structure of Carnitine Transporter from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 124.200, 124.200, 154.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.676 - 3.500 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.302 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.700 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.400 |
| Rmerge | 0.060 | 0.900 |
| Number of reflections | 71232 | |
| <I/σ(I)> | 1.3 | |
| Completeness [%] | 97.7 | 87.1 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
