2WR8
Structure of Pyrococcus horikoshii SAM hydroxide adenosyltransferase in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-29 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 65.561, 65.561, 142.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.780 - 1.770 |
| R-factor | 0.17039 |
| Rwork | 0.168 |
| R-free | 0.21739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wu8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.040 | 0.500 |
| Number of reflections | 22346 | |
| <I/σ(I)> | 11.4 | 3.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 19.83% PEG 6000, 0.26M KCL, 0.1M MOPS PH 7 |
