2WPM
factor IXa superactive mutant, EGR-CMK inhibited
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.200, 66.820, 96.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.700 - 2.000 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wph |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.480 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.710 |
| Number of reflections | 16727 | |
| <I/σ(I)> | 11.5 | 2 |
| Completeness [%] | 83.6 | 68.8 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.85 | 22 % PEG 3000 100 MM BIS/TRIS PH 6.85 |
