2WPI
factor IXa superactive double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.320, 67.350, 98.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 1.990 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wph |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.480 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.160 | 0.430 |
| Number of reflections | 16881 | |
| <I/σ(I)> | 6.5 | 3.1 |
| Completeness [%] | 82.0 | 100 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 24 % PEG 6000 100 MM MES PH 7.1 |
