2WNG

complete extracellular structure of human signal regulatory protein (SIRP) alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-27
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	47.410, 84.370, 98.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.050 - 2.490
R-factor	0.2212
Rwork	0.219
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2UV3 2AJR AND 1ED3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.100	2.550
High resolution limit [Å]	2.490	2.490
Rmerge	0.070	0.850
Number of reflections	14246
<I/σ(I)>	14.2	2
Completeness [%]	98.5	98.2
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5		SITTING DROPS CONTAINING 100 NL SIRP (18.6 MG/ML) PLUS 100 NL PRECIPITANT (1.0 M TRI-SODIUM CITRATE, 0.1 M SODIUM CACODYLATE, PH 6.5) WERE EQUILIBRATED AT 20.5C AGAINST 95 UL RESERVOIRS OF PRECIPITANT. CRYSTALS WERE CRYO-PROTECTED BY A QUICK SWEEP THROUGH PERFLUOROPOLYETHER PFO-X125/03 (LANCASTER SYNTHESIS).