2WNG
complete extracellular structure of human signal regulatory protein (SIRP) alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-27 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.410, 84.370, 98.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.050 - 2.490 |
R-factor | 0.2212 |
Rwork | 0.219 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2UV3 2AJR AND 1ED3 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (2.5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.100 | 2.550 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.070 | 0.850 |
Number of reflections | 14246 | |
<I/σ(I)> | 14.2 | 2 |
Completeness [%] | 98.5 | 98.2 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | SITTING DROPS CONTAINING 100 NL SIRP (18.6 MG/ML) PLUS 100 NL PRECIPITANT (1.0 M TRI-SODIUM CITRATE, 0.1 M SODIUM CACODYLATE, PH 6.5) WERE EQUILIBRATED AT 20.5C AGAINST 95 UL RESERVOIRS OF PRECIPITANT. CRYSTALS WERE CRYO-PROTECTED BY A QUICK SWEEP THROUGH PERFLUOROPOLYETHER PFO-X125/03 (LANCASTER SYNTHESIS). |