2WMC
Crystal structure of eukaryotic initiation factor 4E from Pisum sativum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-30 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.612, 136.320, 74.415 |
Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
Resolution | 136.083 - 2.200 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2idv |
RMSD bond length | 0.011 |
RMSD bond angle | 1.481 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.930 | 2.220 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.240 |
Number of reflections | 72233 | |
<I/σ(I)> | 14.64 | 5.39 |
Completeness [%] | 97.4 | 95.4 |
Redundancy | 3.73 | 3.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 18% (W/V) PEG 3350 IN 100 MM HEPES PH 7.0 AT 293 K |