2WKO
Structure of metal loaded Pathogenic SOD1 Mutant G93A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-26 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.488, 47.157, 56.356 |
Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
Resolution | 56.800 - 1.970 |
R-factor | 0.17666 |
Rwork | 0.174 |
R-free | 0.23072 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c9v |
RMSD bond length | 0.016 |
RMSD bond angle | 1.520 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.580 | 2.030 |
High resolution limit [Å] | 1.970 | 1.960 |
Rmerge | 0.070 | 0.200 |
Number of reflections | 19287 | |
<I/σ(I)> | 13 | 2.4 |
Completeness [%] | 93.6 | 87.9 |
Redundancy | 2.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 20% P3350, 0.2 M AMMONIUM IODIDE, pH 6.5 |