2WIT
CRYSTAL STRUCTURE OF THE SODIUM-COUPLED GLYCINE BETAINE SYMPORTER BETP FROM CORYNEBACTERIUM GLUTAMICUM WITH BOUND SUBSTRATE
Replaces: 2W8AExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | RAYONIX |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.095, 129.422, 182.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.470 - 3.350 |
| R-factor | 0.2572 |
| Rwork | 0.257 |
| R-free | 0.26490 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 3.550 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 37151 | |
| <I/σ(I)> | 23.2 | 1 |
| Completeness [%] | 90.7 | 44.1 |
| Redundancy | 18.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 100 MM NA3CITRATE, PH 5.55, 23% PEG400, 100 MM NACL, 18 CELSIUS |
