2WH6
Crystal structure of anti-apoptotic BHRF1 in complex with the Bim BH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-29 |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.747, 62.747, 92.383 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v6q |
RMSD bond length | 0.015 |
RMSD bond angle | 1.685 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.570 |
Number of reflections | 32170 | |
<I/σ(I)> | 40 | 2.4 |
Completeness [%] | 98.8 | 93.1 |
Redundancy | 9.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 0.1 M MALIC ACID PH 4 1.25 M NABR |