2WH5
Crystal structure of human acyl-CoA binding domain 4 complexed with stearoyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.060, 95.100, 119.740 |
| Unit cell angles | 90.00, 110.28, 90.00 |
Refinement procedure
| Resolution | 42.528 - 2.600 |
| R-factor | 0.2069 |
| Rwork | 0.206 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.481 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.780 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 58524 | |
| <I/σ(I)> | 4.5 | 2 |
| Completeness [%] | 95.0 | 91 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.24M K_THIOCYANATE; 26W/V PEG_3350 |
