2WGP
Crystal structure of human dual specificity phosphatase 14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-16 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 85.011, 85.011, 115.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.880 |
| R-factor | 0.17775 |
| Rwork | 0.175 |
| R-free | 0.22058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2esb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 32950 | |
| <I/σ(I)> | 22.8 | 1.9 |
| Completeness [%] | 99.3 | 93.4 |
| Redundancy | 7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 0.1M SODIUM CITRATE PH 5.4, 1.1 M AMMONIUM PHOSPHATE MONOBASIC,0.1 M NDSB-256 CRYOPROTECTED WITH 20% GLYCEROL |
