2WEI
Crystal structure of the kinase domain of Cryptosporidium parvum calcium dependent protein kinase in complex with 3-MB-PP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.900, 68.900, 130.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.500 - 1.650 |
| R-factor | 0.19716 |
| Rwork | 0.196 |
| R-free | 0.22876 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dfa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.410 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.620 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.110 | 0.700 |
| Number of reflections | 38607 | |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG 3350, 0.1M BIS-TRIS PH 5.5 |
