2WE6
Crystal Structure of Plasmodium falciparum Ubiquitin Carboxyl- terminal Hydrolase 3 (UCHL3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-20 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.343, 44.979, 99.534 |
| Unit cell angles | 90.00, 121.79, 90.00 |
Refinement procedure
| Resolution | 28.210 - 2.420 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wdt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.080 | 0.490 |
| Number of reflections | 24494 | |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 99.2 | 96.2 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 100 MM BICINE PH 9.0, 300 MM MGCL2, 25% PEG 2000, 15% GLYCEROL |
