2WDW
The Native Crystal Structure of the Primary Hexose Oxidase (Dbv29) in Antibiotic A40926 Biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-25 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 66.092, 66.092, 790.462 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.540 - 3.210 |
R-factor | 0.24 |
Rwork | 0.237 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ipi |
RMSD bond length | 0.005 |
RMSD bond angle | 0.837 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.320 |
High resolution limit [Å] | 3.210 | 3.210 |
Rmerge | 0.080 | 0.160 |
Number of reflections | 17824 | |
<I/σ(I)> | 24.8 | 17 |
Completeness [%] | 95.7 | 92 |
Redundancy | 9.7 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 30% PEG 550 MME, 0.05M CALCIUM CHLORIDE, 0.1M BIS TRIS, PH 6.5 |