2WC0
crystal structure of human insulin degrading enzyme in complex with iodinated insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2007-04-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 263.169, 263.169, 90.875 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.850 - 2.800 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g47 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.515 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.530 |
| Number of reflections | 97980 | |
| <I/σ(I)> | 19.8 | 3.6 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 6.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
