2WBU
CRYSTAL STRUCTURE OF THE ZINC FINGER DOMAIN OF KLF4 BOUND TO ITS TARGET DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.275, 50.275, 131.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.500 |
| R-factor | 0.22 |
| Rwork | 0.214 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wbs |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.355 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 6277 | |
| <I/σ(I)> | 20.4 | 4.2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 8.3 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 27 % PEG 3350, 0.2 M NAACETATE, 0.1 M TRIS-HCL PH 8.5 |
