2WBF
Crystal Structure Analysis of SERA5E from plasmodium falciparum with loop 690-700 ordered
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-21 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.360, 102.360, 71.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.550 - 1.600 |
| R-factor | 0.153 |
| Rwork | 0.152 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ch2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.833 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.670 |
| Number of reflections | 36811 | |
| <I/σ(I)> | 24.6 | 2.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 17% PEG3350,0.2M CACL2, 5% DMSO, 10MM BIS-TRIS PH 6.5, 10MM NACL, SITTING-DROP VAPOUR-DIFFUSION |
