2WAT
Structure of the fungal type I FAS PPT domain in complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.587, 73.764, 105.689 |
| Unit cell angles | 90.00, 130.80, 90.00 |
Refinement procedure
| Resolution | 80.010 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.040 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 43185 | |
| <I/σ(I)> | 7.8 | 2.3 |
| Completeness [%] | 97.9 | 89 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | pH 4 |
