2WA6
Structure of the W148R mutant of human filamin b actin binding domain at 1.95 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.031, 69.304, 89.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.370 - 1.950 |
| R-factor | 0.19734 |
| Rwork | 0.195 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wa5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.247 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.680 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 21524 | |
| <I/σ(I)> | 18.3 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 10% PEG 6000, 5% MPD, 0.1 M HEPES PH 7.5. |
