2WA2

Structure of the methyltransferase domain from Modoc Virus, a Flavivirus with No Known Vector (NKV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	52.530, 62.242, 160.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.18828
Rwork	0.186
R-free	0.23479
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l9k
RMSD bond length	0.015
RMSD bond angle	1.384
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.320
Number of reflections	45634
<I/σ(I)>	21.5	4.1
Completeness [%]	92.0	63.7
Redundancy	6.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1