2WA0
Crystal structure of the human MAGEA4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 81.630, 81.630, 210.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.810 - 2.300 |
| R-factor | 0.24434 |
| Rwork | 0.243 |
| R-free | 0.27413 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.540 |
| Number of reflections | 19314 | |
| <I/σ(I)> | 13.9 | 2.2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2.6 M NACL, 0.1 M TRIS PH 8 | ||
| 1 | 8 | 2.6 M NACL, 0.1 M TRIS PH 8 |
