2W9E
Structure of ICSM 18 (anti-Prp therapeutic antibody) Fab fragment complexed with human Prp fragment 119-231
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-07 |
Detector | MARRESEARCH |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 126.149, 126.149, 134.126 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uw3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.254 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.140 | 0.670 |
Number of reflections | 13209 | |
<I/σ(I)> | 12 | 2 |
Completeness [%] | 86.2 | 70 |
Redundancy | 8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | AMMONIUM SULPHATE, TRIS PH 8.0 |