2W8M
Structure of D212, a nuclease from a fusselovirus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-21 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.85, 2.06 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.477, 81.971, 60.160 |
Unit cell angles | 90.00, 115.40, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.400 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24500 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.005 |
RMSD bond angle | 0.698 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.030 | 0.330 |
Number of reflections | 17143 | |
<I/σ(I)> | 32 | 4.3 |
Completeness [%] | 92.9 | 95.7 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES (PH 7.5), 0.05 M K2HPO4, 10% (W/V) PEG 8000 WITH 25% GYLCEROL AS CRYOPROTECTANT | ||
1 | 7.5 | 0.1 M HEPES (PH 7.5), 0.05 M K2HPO4, 10% (W/V) PEG 8000 WITH 25% GYLCEROL AS CRYOPROTECTANT |