2W86
Crystal structure of fibrillin-1 domains cbEGF9hyb2cbEGF10, calcium saturated form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-13 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.232, 47.258, 89.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.750 - 1.800 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.22120 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.027 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.790 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.470 |
Number of reflections | 15382 | |
<I/σ(I)> | 24.3 | 7.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.3 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 100 MM TRIS PH 7.5, 200 MM NAI, 16% PEG 3350 |