2W57

Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals structural rearrangement of the DNA-binding domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Detector	RIGAKU SATURN 944
Spacegroup name	P 43 21 2
Unit cell lengths	88.880, 88.880, 85.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.870 - 2.600
R-factor	0.218
Rwork	0.216
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1MZB 2fu4
RMSD bond length	0.010
RMSD bond angle	1.215
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.600
Number of reflections	10961
<I/σ(I)>	16.6	3.3
Completeness [%]	100.0	100
Redundancy	8.6	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1