2W57
Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals structural rearrangement of the DNA-binding domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.880, 88.880, 85.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.870 - 2.600 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1MZB 2fu4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.215 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.130 | 0.600 |
Number of reflections | 10961 | |
<I/σ(I)> | 16.6 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.6 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |