2W41
Crystal structure of Plasmodium falciparum glycerol kinase with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.529, 57.632, 123.922 |
| Unit cell angles | 90.00, 89.75, 90.00 |
Refinement procedure
| Resolution | 124.030 - 2.410 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bu6 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.030 | 2.470 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.100 | 0.840 |
| Number of reflections | 39660 | |
| <I/σ(I)> | 10.95 | 0.71 |
| Completeness [%] | 91.4 | 40.5 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 50MM KFORMATE, 20% PEG 3350, 25% ETHYLENE GLYCOL, 10MM LDAO, 20MM GLYCEROL, THEN SOAKED WITH 5 MM ADP AND 10 MM MGCL2, pH 7.5 |
