2W36
Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 51.979, 132.239, 191.616 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.25800 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.350 |
Number of reflections | 32087 | |
<I/σ(I)> | 69 | 5.9 |
Completeness [%] | 87.6 | 62 |
Redundancy | 13.3 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |