2W2W

PLCg2 Split Pleckstrin Homology (PH) Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-13
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	92.216, 106.017, 194.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.050 - 2.800
R-factor	0.306
Rwork	0.303
R-free	0.35200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1upq
RMSD bond length	0.010
RMSD bond angle	1.226
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	3.480
High resolution limit [Å]	3.300	3.300
Rmerge	0.090	0.560
Number of reflections	28821
<I/σ(I)>	7.1	1.4
Completeness [%]	98.4	95.2
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	293	PLCSPPH(Y495F) WAS CRYSTALLIZED USING A PROTEIN CONCENTRATION OF 36 MG/ML WITH PRECIPITANT (20% PEG3350, 100 MM BIS-TRIS PROPANE PH 7.5) AT A CONSTANT TEMPERATURE OF 20C