2W2O
PCSK9-deltaC D374Y mutant bound to WT EGF-A of LDLR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.948, 85.948, 218.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.620 |
| R-factor | 0.22843 |
| Rwork | 0.227 |
| R-free | 0.26311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qtw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.238 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.760 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.080 | 0.700 |
| Number of reflections | 25321 | |
| <I/σ(I)> | 14.2 | 2.3 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 10% (W/V) PEG 8000 AND 8% (V/V) ETHYLENE GLYCOL |
