2W2M
WT PCSK9-DELTAC BOUND TO WT EGF-A OF LDLR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.004, 83.004, 211.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.400 |
| R-factor | 0.21719 |
| Rwork | 0.215 |
| R-free | 0.25421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qtw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.228 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.680 |
| Number of reflections | 29902 | |
| <I/σ(I)> | 13.4 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 10% (V/V) 2-PROPANOL, 20% (W/V) PEG 4000 |
