2W2J
Crystal structure of the human carbonic anhydrase related protein VIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.267, 73.854, 87.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.860 - 1.600 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.123 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.990 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.580 |
| Number of reflections | 38029 | |
| <I/σ(I)> | 11.5 | 1.8 |
| Completeness [%] | 98.1 | 87.2 |
| Redundancy | 6.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 20 MM NH4CL, 20.0% PEG 6K, 10.0% GLYCEROL, pH 6.3 |
