2W2I

Crystal structure of the human 2-oxoglutarate oxygenase LOC390245

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-04
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	90.904, 111.009, 224.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	111.800 - 2.100
R-factor	0.212
Rwork	0.210
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oq7
RMSD bond length	0.012
RMSD bond angle	1.326
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0055)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.490
Number of reflections	63031
<I/σ(I)>	10.1	1.8
Completeness [%]	99.9	99.4
Redundancy	4.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1